Working with stock solutions
  1. The recommended buffer for Hind III is
    • 10 mM Tris-HCl (pH 8.5),
    • 10 mM MgCl2,
    • 100 mM KCl, and
    • 0.1 mg/ml BSA
    What would be the BSA concentration in a 100X stock buffer?

    1 mg/ml

    0.1 mg/ml

    0.1 g/ml

    10 mg/ml

    100 ug/ml


  2. The recommended buffer for Hind III is
    • 10 mM Tris-HCl (pH 8.5),
    • 10 mM MgCl2,
    • 100 mM KCl, and
    • 0.1 mg/ml BSA
    What would be the KCl concentration in a 10X stock buffer?

    10 mM

    0.01 M

    1 M

    10 mM


  3. If you were setting up a 15 µl restriction digest using Hind III, how much of the 10X stock buffer would you use (assume final should be 1X)?

    15 µl

    1.5 µl

    0.15 ml

    0.15 µl


  4. Every couple of years I make a 50X TAE stock (gel running buffer). For running gels I dilute this stock to 1X in 4 L quantities.
    How much 50X TAE do I use each time I make 4 L of 1X TAE?

    8 ml

    80 ml

    200 ml

    50 ml

    20 ml


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